Principles and Applications of Creatinine Measurement Using the TOPS Reagent 40567-80-4 SOX Method

In the field of clinical testing and biochemical analysis, accurate determination of creatinine content is of great significance for evaluating physiological indicators such as renal function. Among them, TOPS reagent SOX method is one of the widely used technologies, which provides strong support for related detection work with stable reaction processes and reliable detection results. The following will provide a detailed analysis of the method from the aspects of measurement principle, method comparison, and reagent supply.

1, The core principle of TOPS reagent SOX method for determining creatinine

The TOPS reagent SOX method is used to determine creatinine, which relies on TOPS as the substrate and the catalytic effect of creatine oxidase (SOX) to achieve the detection of creatinine content. The entire process requires multiple enzymatic reactions to be completed in synergy.

TOPS powder

Firstly, creatinine undergoes a hydrolysis reaction under the specific catalysis of creatine kinase (creatinine aminohydrolase) and is converted into creatine. This step of the reaction is the starting point of the entire detection process, and the catalytic activity of creatinine enzyme directly affects the efficiency of subsequent reactions. Its specificity for creatinine ensures that the reaction will not be interfered by other similar substances, laying the foundation for the accuracy of the detection.

Subsequently, the generated creatine is further hydrolyzed by creatine kinase (CRH, creatine amidine hydrolase) to produce creatine and urea. The participation of creatine kinase enables creatine to be smoothly broken down into the key substance required for subsequent reactions - creatine, while producing urea as a byproduct of the reaction, providing a basis for possible comparative testing in the future.

Subsequently, creatine undergoes an oxidation reaction under the catalysis of creatine oxidase (SOX), producing glycine, formaldehyde, and hydrogen peroxide. This step is a key reaction in the entire detection process, and the amount of hydrogen peroxide generated is directly related to the initial concentration of creatinine, becoming an important indicator for subsequent quantitative detection.

Finally, the generated hydrogen peroxide undergoes a color reaction with TOPS (N-ethyl-N - (2-hydroxy-3-sulfopropyl) -3,5-dimethoxyaniline) and 4-AAP (4-aminoantipyrine) in the presence of peroxidase, forming a stable color product. By measuring the absorbance of the colorimetric product using a spectrophotometer, the specific content of creatinine in the sample can be calculated based on the measured absorbance value, as the absorbance is directly proportional to the content of the colorimetric product, which in turn is directly proportional to the initial concentration of creatinine.

2, Comparison between TOPS reagent SOX method and urea detection method

The creatinine determination method using TOPS as a chromogenic substrate has shown strong practicality in practical applications and has become one of the commonly used detection methods. It is worth noting that creatine and urea are generated during the reaction process of TOPS reagent SOX method. Based on this characteristic, creatinine content can also be indirectly measured through urea detection method.

The principle of urea detection method is to calculate the concentration of creatinine by detecting the urea content generated during the reaction process and combining it with the stoichiometric relationship of the reaction. However, compared with the TOPS reagent SOX method, the urea detection method has significant shortcomings in sensitivity. Due to the indirect calculation of creatinine concentration required by the urea detection method, it may be affected by various factors during the process, such as the influence of other substances in the reaction system on urea detection, or the accumulation of errors in the calculation process, which makes it difficult for the accuracy and sensitivity of its detection results to reach the level of TOPS reagent SOX method. Therefore, in scenarios where high detection sensitivity is required, TOPS reagent SOX method is still a better choice.

3, Supply support for reagents required for creatinine testing

The smooth implementation of creatinine testing relies on the guarantee of high-quality reagents. As a professional reagent supplier, Desheng can provide various key reagents required in the creatinine detection process, covering multiple categories such as chromogens, enzymes, buffers, etc., providing comprehensive supply support for detection institutions and related research units.

Product packaging

With a wide range of reagent categories and stable product quality, Desheng provides strong material support for creatinine detection work, helping to improve detection efficiency and result accuracy, and providing reliable guarantees for clinical diagnosis and scientific research.