Enzyme based biologics are playing an increasingly crucial role in contemporary medical diagnosis and health management. Among them, uricase (Uricase, EC1.7.3.3) has become a shining star in in vitro diagnosis and disease treatment due to its unique catalytic mechanism and wide application prospects. It not only promotes the development of precision medical diagnosis, but also plays an irreplaceable role in multiple industrial and health scenarios.
Combining natural and recombinant technologies: Breaking through limitations and improving performance
Uric acid enzyme, also known as uric acid oxidase, can efficiently catalyze the oxidation of uric acid to produce high solubility and easily excreted uric acid. Natural uricase is extracted from microorganisms such as Aspergillus niger and Aspergillus flavus through fermentation, but it has problems such as strong immunogenicity and poor stability. Through genetic engineering technology, recombinant uricase has successfully overcome these bottlenecks - not only reducing the risk of adverse reactions, but also significantly enhancing the stability and catalytic efficiency of the enzyme. This technological breakthrough enables uricase to maintain activity in a wider range of environments, laying a solid foundation for its diversified applications.
Core advantage: catalytic ability with high specificity and sensitivity
The most unique feature of uricase is its highly specific catalytic performance. It can work efficiently around pH 7.5 and is highly compatible with the human physiological environment. Although enzyme activity can be interfered by inhibitors such as cyanide, Ag ⁺, Hg ² ⁺, and is prone to deactivation above 40 ℃, these characteristics actually make it exhibit excellent controllability and accuracy in strictly controlled detection scenarios.
For example, in in in vitro diagnostics, uricase catalyzes the generation of H ₂ O ₂ from uric acid, which is then detected by fluorescence or colorimetric methods to achieve extremely accurate quantification of uric acid concentration. This mechanism avoids the interference of non-specific reactions and greatly improves the reliability of detection results.
Core application scenarios: from medical diagnosis to industrial monitoring
1. Medical diagnosis: screening and management of hyperuricemia and gout
The largest application of uricase is in uric acid detection kits and fully automated biochemical analyzers. It is the gold standard tool for measuring serum, plasma, or urine uric acid concentrations in hospitals and testing centers. For patients with hyperuricemia and gout, regular monitoring of uric acid levels is crucial. The uricase kit has the characteristics of high throughput, rapid response, and low error, making it very suitable for daily use in large medical institutions.
2. Food and Textile Industry: Process Monitoring and Quality Assurance
Uric acid, as a metabolic product, may accumulate in certain foods (such as meat and seafood) and industrial fermentation processes, affecting product safety and quality. Urinase can be used to construct biosensors for real-time monitoring of uric acid levels in the production process, assisting enterprises in achieving automation and refinement of quality control. For example, in fields such as beer brewing and seafood processing, such applications are gradually becoming popular.
3. Health Management and Home Testing
With the development of microfluidic technology and portable detection devices, uricase has also been integrated into miniaturized detection chips or test strips, suitable for home or personal health management. Users can monitor their uric acid levels in real-time with just one drop of blood, making it particularly suitable for daily monitoring of gout susceptible individuals.
Hubei Xindesheng Material Technology Co., Ltd. has established a diagnostic enzyme research and development team, which is currently capable of independently producing dozens of enzyme preparations such as uricase for in vitro diagnostic kits. The products have good quality and stable performance, and are highly welcomed by users. If you have any recent purchasing needs, please click on the official website to learn more details or contact me!
