Dipotassium ethylenediaminetetraacetate is abbreviated as EDTA-2K. Its appearance is white crystalline powder. It is soluble in water and slightly soluble in alcohol. Its aqueous solution has a pH of about 5.3 and a melting point of 272 °C. Dipotassium ethylenediaminetetraacetate has a wide range of uses. It can be used to complex metal ions and separate metals. It is also used in detergents, liquid soaps, shampoos, agricultural chemical sprays, antidotes, and is commonly used in blood anticoagulants.
Dipotassium ethylenediaminetetraacetate (EDTA-2K)
Dipotassium ethylenediaminetetraacetate can be used for complete blood counts. Whole blood cell analysis is widely used in clinical testing. Platelet count has become an important basis for the diagnosis and treatment of clinical thrombosis and bleeding diseases, and it is also one of the important parameters for preoperative detection of surgical patients. The International Committee for Blood Standards recommends the use of dipotassium ethylenediaminetetraacetate (EDTA-K2) anticoagulant blood for complete blood counts.
Studies have also explored the detection of total bilirubin (TBIL), direct bilirubin (DBIL), total protein (TP), albumin (ALB), and alanine in anticoagulated plasma and serum of dipotassium ethylenediaminetetraacetate. Differences in the results of 8 liver function indexes including aminotransferase (ALT), aspartate aminotransferase (AST), γ-glutamyltransferase (GGT), alkaline phosphatase (ALP) and so on. Methods The above eight biochemical tests were performed on EDTA-K2 anticoagulated plasma and serum on an automatic biochemical analyzer, and the results were compared and evaluated.
The results showed that EDTA-K2 anticoagulated plasma compared with serum, the results of TBIL, TP, ALB, ALT, AST were not statistically significant, EDTA-K2 anticoagulated plasma ALP was significantly lower than that of serum, the difference was statistically significant, and GGT was slightly lower In serum, the difference was statistically significant, and DBIL was significantly higher than that in serum, and the difference was statistically significant. Therefore, EDTA-K2 anticoagulated plasma can be used for the detection of TBIL, TP, ALB, ALT, and AST. It is not suitable for the detection of DBIL and ALP. For the GGT test results, the reference range of EDTA-K2 plasma should be formulated or multiplied by the correction factor.
Application of dipotassium ethylenediaminetetraacetate (EDTA-2K):
1. Additives for vacuum blood collection tubes: The additive of vacuum blood collection tubes is an aqueous solution of dipotassium ethylenediaminetetraacetate (EDTA-2K), and 4mg of dipotassium ethylenediaminetetraacetate (EDTA-2K) is required for anticoagulation of 2ml of blood. Because the concentration is small, in order not to dilute the blood, 20μl of an aqueous solution containing 200g/L of ethylenediaminetetraacetate (EDTA-2K) 200g/L is generally pre-installed in each blood collection tube. The validity period of the blood collection tube is two years. When the operating environment changes slightly, such as temperature changes, water can easily evaporate to the pipe wall (especially the water in the plastic PET pipe solvent can penetrate the pipe wall and leak out), causing the EDTA-2K in the pipe to crystallize and crystallize quickly When collecting blood, the latter dipotassium ethylenediaminetetraacetic acid blood collection tube is required to be turned upside down at least 8 times in order to fully dissolve and mix the crystallized EDTA-2K in the blood. If the action is too large, the red blood cells will be destroyed and hemolyzed. Gathered and adhered and broken.
2. Prepare toilet deodorant. In parts by mass, it includes the following substances: 5-10 parts of acid salt, appropriate amount of ammonia, 50-70 parts of alcohol, 5-10 parts of urotropine, appropriate amount of dipotassium edetate, appropriate amount of isobutanolamine, water 60 to 80 copies. The deodorant can not only deodorize but also prevent scale, and the method of use is simple, the dosage is small, and the duration is long, and it has no corrosive effect on the toilet. The production method is simple, the raw material cost is low, and it is non-toxic, harmless and non-polluting.
3. Used as a complexing agent in liquid phase analysis. If the invention provides a tetrabutylammonium hydrogen sulfate buffer salt system for liquid chromatography detection, the solvent is water, and the solute includes tetrabutylammonium hydrogen sulfate and a buffer ion pair. The buffer ion pair is composed of potassium dihydrogen phosphate and phosphoric acid The solute also includes a complexing agent, and the complexing agent is preferably dipotassium ethylenediaminetetraacetate.
The tetrabutylammonium hydrogen sulfate buffer salt system provided above is stable within 24 hours without turbidity. Taking the analysis of related substances of moxifloxacin hydrochloride as an example, it can be seen that the tetrabutylammonium hydrogen sulfate buffer salt system stored at room temperature for 24 hours The chromatographic behavior of moxifloxacin, N-methyl impurity, impurity A, impurity B, impurity C, impurity D, and impurity E on the reversed-phase chromatography column is basically the same as that of the newly configured tetrabutylammonium hydrogen sulfate buffer salt system.
4. Prepare metal polishing agent. The polishing agent is based on dipotassium ethylenediaminetetraacetate (EDTA-2K), trisodium phosphate, dibutyl phthalate, sodium silicate, inorganic acid, dicarboxylic acid compound, tetrapolyricinoleate, biological Buffer, stabilizer, water-soluble surfactant, water as raw materials, and through a scientific content ratio, the polishing agent prepared from this can effectively remove impurities on the metal surface. It is not only convenient to operate, but also has a good polishing effect. Improve the production efficiency, and ensure the cleanliness and gloss of the metal surface, and improve the quality of the metal.
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