가정
제품
저희에 관하여
공장 투어
품질 관리
저희에게 연락하십시오
견적 요청
뉴스
제품 소개트리스(하이드록시메틸)아미노메탄

단백질 SDS-PAGE 전기영동을 위한 CAS 77-86-1 트리스 염산 버퍼

중국 Wuhan Desheng Biochemical Technology Co., Ltd 인증
중국 Wuhan Desheng Biochemical Technology Co., Ltd 인증
병원 대리인의 분배자로, 당신의 혈액 수집 관 첨가물은 우리가 서로 좋은 사업을 설치하는 것을 가지고 있다는 것을 아주 나의 필요를 위한 한 벌, 나 생각합니다입니다, 당신을 감사하십시오!

—— 토니

제가 지금 온라인 채팅 해요

단백질 SDS-PAGE 전기영동을 위한 CAS 77-86-1 트리스 염산 버퍼

CAS 77-86-1 Tris Hydrochloric Acid Buffer For Protein SDS-PAGE Electrophoresis
CAS 77-86-1 Tris Hydrochloric Acid Buffer For Protein SDS-PAGE Electrophoresis CAS 77-86-1 Tris Hydrochloric Acid Buffer For Protein SDS-PAGE Electrophoresis CAS 77-86-1 Tris Hydrochloric Acid Buffer For Protein SDS-PAGE Electrophoresis CAS 77-86-1 Tris Hydrochloric Acid Buffer For Protein SDS-PAGE Electrophoresis CAS 77-86-1 Tris Hydrochloric Acid Buffer For Protein SDS-PAGE Electrophoresis

큰 이미지 :  단백질 SDS-PAGE 전기영동을 위한 CAS 77-86-1 트리스 염산 버퍼

제품 상세 정보:

원래 장소: 후베이 성, 중국
브랜드 이름: DESHENG
인증: ISO9001
모델 번호: TRIS

결제 및 배송 조건:

최소 주문 수량: 500g
가격: Consult the latest quotation
포장 세부 사항: 500g / 병, 25kg/배럴
배달 시간: 5 일
지불 조건: L/C, D/P, T/T, 서부 동맹
공급 능력: 달 당 100T
상세 제품 설명
이름: TRIS CAS 번호: 77-86-1
분자량: 121.135 분자식: C4H11NO3
출현: 백색 수정같은 분말 저장 상태: 실내 온도, 광선이 안 통하고는 방습
하이 라이트:

CAS 77-86-1 Tris Hydrochloric Acid Buffer

,

PH 7.5 Tris Hydrochloric Acid Buffer

,

77-86-1 tris hcl ph 8

Tris hydrochloric acid buffer for protein SDS-PAGE electrophoresis;CAS 77-86-1;Buffer Solution;White crystal powder

 

Tris hydrochloric acid buffer for protein SDS-PAGE electrophoresis


Tris hydrochloride is tris-hydroxymethylamine-hydrochloride Tris-HCl buffer, which is suitable for most protein and nucleic acid-related research experiments. It is usually used as a buffer for protein SDS-PAGE electrophoresis experiments with good electrophoresis. High-resolution.
Biological buffer composed of Tris, often prepared with pH values ​​of 6.8, 7.4, 8.0, 8.8. Its pH value changes relatively greatly with temperature. Generally speaking, the pH value decreases by 0.03 for every degree of temperature increase. 1M Tris-HCl 6.8 and 1.5M Tris-HCl 8.8 are the most commonly used reagents for SDS-PAGE. Tris can dissolve nucleic acids and proteins.

Configuration of Tris buffer solution

PH value required(25℃) 7.10 7.20 7.30 7.40 7.50 7.60 7.70 7.80 7.90 8.00 8.10 8.20 8.30 8.40 8.50 8.60 8.70 8.80 8.90
0.1mol/L HCI volume 45.7 44.7 43.4 42 40.3 38.5 36.6 34.5 32 29.2 26.2 22.9 19.9 17.2 14.7 12.4 10.3 8.5 7
 

 

단백질 SDS-PAGE 전기영동을 위한 CAS 77-86-1 트리스 염산 버퍼 0

Tris hydrochloric acid buffer raw material
SDS-PAGE is a fairly common polyacrylamide gel electrophoresis. It uses polyacrylamide gel as a supporting medium and is suitable for the separation of proteins and oligonucleotides. Polyacrylamide gels have a microscopic network structure and have a molecular sieve effect. They are divided into two forms: non-denaturing polyacrylamide gel electrophoresis (Native-PAGE) and SDS-polyacrylamide gel (SDS-PAGE).
In the protein SDS-PAGE discontinuous electrophoresis experiment, the gel preparation buffer used the Tris-HCL buffer system, the concentrated gel had a pH of 6.7, and the separation gel had a pH of 8.9; and the electrophoresis buffer used the Tris-glycine buffer system. In the concentrated electrophoresis gel, its pH value is weakly acidic, so only a small amount of glycine will dissociate. Under the action of an electric field, its migration efficiency is low; while the chloride ion is very high, forming a conduction between the two In the lower sex zone, the protein molecule moves between the two. Since the conductivity is inversely proportional to the electric field strength, this zone forms a higher voltage gradient, pressing the protein molecules together and condensing into a narrow zone.

단백질 SDS-PAGE 전기영동을 위한 CAS 77-86-1 트리스 염산 버퍼 1
When the sample enters the separation gel, due to the increase of pH in the gel, it becomes alkaline. The glycine dissociates in a large amount, and the migration rate increases. It directly follows the chloride ion. At the same time, due to the shrinkage of the separation gel pore size, the protein Molecules are separated according to their inherent chargeability and molecular size.
Therefore, the influence of pH on the entire reaction system is very important. If the problem cannot be solved well after excluding other factors in the experiment, this factor should be considered first. Of course, other factors can also be considered from many aspects.
Tris hydrochloric acid is made by mixing Tris and hydrochloric acid, such as tromethamine, tromethamine, 2-amino-2-(hydroxymethyl)-1,3-propanediol, etc., all refer to Tris, which is produced by Desheng Important raw materials, can also be used for other various buffer systems.

단백질 SDS-PAGE 전기영동을 위한 CAS 77-86-1 트리스 염산 버퍼 2

연락처 세부 사항
Wuhan Desheng Biochemical Technology Co., Ltd

담당자: Sales Manager

회사에 직접 문의 보내기 (0 / 3000)