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CAS 77-86-1 Tris Hydrochloric Acid Buffer,
PH 7.5 Tris Hydrochloric Acid Buffer,
77-86-1 tris hcl ph 8
Tris hydrochloric acid buffer for protein SDS-PAGE electrophoresis；CAS 77-86-1；Buffer Solution；White crystal powder
Tris hydrochloric acid buffer for protein SDS-PAGE electrophoresis
Tris hydrochloride is tris-hydroxymethylamine-hydrochloride Tris-HCl buffer, which is suitable for most protein and nucleic acid-related research experiments. It is usually used as a buffer for protein SDS-PAGE electrophoresis experiments with good electrophoresis. High-resolution.
Biological buffer composed of Tris, often prepared with pH values of 6.8, 7.4, 8.0, 8.8. Its pH value changes relatively greatly with temperature. Generally speaking, the pH value decreases by 0.03 for every degree of temperature increase. 1M Tris-HCl 6.8 and 1.5M Tris-HCl 8.8 are the most commonly used reagents for SDS-PAGE. Tris can dissolve nucleic acids and proteins.
Configuration of Tris buffer solution
|PH value required(25℃)||7.10||7.20||7.30||7.40||7.50||7.60||7.70||7.80||7.90||8.00||8.10||8.20||8.30||8.40||8.50||8.60||8.70||8.80||8.90|
|0.1mol/L HCI volume||45.7||44.7||43.4||42||40.3||38.5||36.6||34.5||32||29.2||26.2||22.9||19.9||17.2||14.7||12.4||10.3||8.5||7
Tris hydrochloric acid buffer raw material
SDS-PAGE is a fairly common polyacrylamide gel electrophoresis. It uses polyacrylamide gel as a supporting medium and is suitable for the separation of proteins and oligonucleotides. Polyacrylamide gels have a microscopic network structure and have a molecular sieve effect. They are divided into two forms: non-denaturing polyacrylamide gel electrophoresis (Native-PAGE) and SDS-polyacrylamide gel (SDS-PAGE).
In the protein SDS-PAGE discontinuous electrophoresis experiment, the gel preparation buffer used the Tris-HCL buffer system, the concentrated gel had a pH of 6.7, and the separation gel had a pH of 8.9; and the electrophoresis buffer used the Tris-glycine buffer system. In the concentrated electrophoresis gel, its pH value is weakly acidic, so only a small amount of glycine will dissociate. Under the action of an electric field, its migration efficiency is low; while the chloride ion is very high, forming a conduction between the two In the lower sex zone, the protein molecule moves between the two. Since the conductivity is inversely proportional to the electric field strength, this zone forms a higher voltage gradient, pressing the protein molecules together and condensing into a narrow zone.
When the sample enters the separation gel, due to the increase of pH in the gel, it becomes alkaline. The glycine dissociates in a large amount, and the migration rate increases. It directly follows the chloride ion. At the same time, due to the shrinkage of the separation gel pore size, the protein Molecules are separated according to their inherent chargeability and molecular size.
Therefore, the influence of pH on the entire reaction system is very important. If the problem cannot be solved well after excluding other factors in the experiment, this factor should be considered first. Of course, other factors can also be considered from many aspects.
Tris hydrochloric acid is made by mixing Tris and hydrochloric acid, such as tromethamine, tromethamine, 2-amino-2-(hydroxymethyl)-1,3-propanediol, etc., all refer to Tris, which is produced by Desheng Important raw materials, can also be used for other various buffer systems.
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