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Wuhan Desheng Biochemical Technology Co., Ltd
Wuhan Desheng Biochemical Technology Co., Ltd
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CAS 1135-40-6 좋은 완충 용액은 시클로헥실아민 프로판술폰산을 완성합니다

제품 세부 정보

원래 장소: 중국

브랜드 이름: De Sheng

인증: ISO9001

모델 번호: 모자

지불 및 배송 조건

최소 주문 수량: 10g

가격: Negotiable

포장 세부 사항: 플라스틱 병 / 용지함

배달 시간: 1-3 일

지불 조건: L/C (신용장), 인수 인도, D/P (지급도 조건), 전신환, 웨스턴 유니온, 머니그램

공급 능력: 100KG/Month

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CAS 1135-40-6 좋은 완충 용액이 시클로헥실아민 프로판술포닉 Acid

,

99% 시클로헥실아민 프로판술폰산을 완성합니다

,

99% Cyclohexylamine Propanesulfonic Acid

항목:
모자
출현:
백색 결정
패키지:
플라스틱 병
CAS#:
1132-61-2년
MOQ:
10g
애플리케이션:
생화학 버퍼
항목:
모자
출현:
백색 결정
패키지:
플라스틱 병
CAS#:
1132-61-2년
MOQ:
10g
애플리케이션:
생화학 버퍼
설명
CAS 1135-40-6 좋은 완충 용액은 시클로헥실아민 프로판술폰산을 완성합니다

Application Of CAPS Cyclohexylamine Propanesulfonic Acid CAS1135-40-6 In WB Experiment

 

Brief introduction

Full Name N-Cyclohexyl-3-aminopropanesulfonic acid Molecular weight 221.32
Abbreviation CAPS Molecular formula C9H19NO3S
CAS No 1135-40-6 ENIECS 214-492-1
Appearance White crystal powder Purity ≥99%

 

Molecular Structure

CAS 1135-40-6 좋은 완충 용액은 시클로헥실아민 프로판술폰산을 완성합니다 0

Good's buffer, which is zwitterionic buffer. This type of buffer is generally a sulfamic acid-based organic compound. After the solution is formulated, the pH is relatively stable and does not react with various enzymes and chemical reagents. It is mainly used in biology and medicine.
 
Good’s buffer has the following general characteristics
(1) The solubility in water is high;
(2) acid and alkali dissociation constant (pKa value) is stable, generally in 6~8, the ability to release protons is stable, and little affected by the ion concentration, solution composition and temperature;
(3) has a low cell membrane permeability, it is not easy to penetrate the biofilm;
(4) can chelate with metal ions in low molar mass, but not generate precipitation with metal ions;
(5) Chemical stability is high;
(6) the light absorption in the ultraviolet and visible wavelength ranges is small;
(7) the possibility of salting out with the organic macromolecules in the organism is small;
(8) the high purity salt is easy to obtain; (9) does not participate in the biochemical reaction process, does not interfere with the biochemical reaction process.
At present, the commonly used Good's buffers are shown in the following table, in which the concentration of buffer and sodium hydroxide is 0.1mol/L.
 

Application of CAPS

At present, the PVD transmembrane of protein has not used Tris-gly, SDS, 20% methanol buffer, because there is glycine in the buffer, so it is difficult to distinguish whether glycine is brought in by Buffer or the protein itself when sequencing. Now the transmembrane buffer used for sequencing is 10 mM/1 CAPS 10% methanol, and the methanol concentration range in CAPS imprint buffer is 0-20%. In general, the buffer with high methanol concentration is paired. The transfer efficiency of low molecular weight proteins is good, while the buffer with low or no methanol concentration is beneficial to the transfer of high molecular weight proteins.

 

Advantage of CAPS

The purity (> 99%) is water-soluble, the process is stable, and the appearance of the product can be guaranteed to be pure white crystal powder.

 

Preparation of CAPS

prepare 0.1mol/L buffer solution with the volumetric flask. During the configuration process, the buffer needs high temperature dehydration, and cooling in the dryer; preparation of 0.1mol/L sodium hydroxide solution. According to the formula in the above table, the corresponding pH buffer can be obtained, sodium hydroxide is mainly used to adjust the pH.

 

CAS 1135-40-6 좋은 완충 용액은 시클로헥실아민 프로판술폰산을 완성합니다 1

 

CAS 1135-40-6 좋은 완충 용액은 시클로헥실아민 프로판술폰산을 완성합니다 2

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